Article Snippet: ZO‐1 knockdown was performed by transiently transfected cells with a mixture containing 5 µg of mZO1‐1 shRNA, a gift from Alan Fanning (Addgene plasmid # 37215), plus 5 µl of Lipofectamine 2000 (Thermo Fisher Scientific) or the empty vector pLL5.0 (kindly provided by Christina Van Itallie and James Anderson from The Laboratory of Tight Junction Structure and Function, National Heart, Lung, and Blood Institute, National Institutes of Health), plus 5 µl of Lipofectamine ...
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When cells reached 80% confluent, they were transfected with SOCS-3 expression plasmids using Lipofectamine 2000 plus reagent according to the manufacturer’s instructions. PRK5 were used as a control. Successful transfection of SOCS-3 genes into trophoblasts was confirmed by positive green fluorescence reviewed under fluorescent microscope. Lipofectamine® 2000 Reagent Protocol 2013-2-Lipofectamine® 2000 DNA Transfection Reagent Protocol Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations. 上海代轩生物科技有限公司发布在丁香通的Lipofectamine® 3000 Transfection Reagent报价、型号、品牌等供应信息介绍，丁香通致力为您提供最优质的Lipofectamine® 3000 Transfection Reagent厂商信息。
They were trans- fected in cells using Lipofectamine 2000 plus 5 μg/mL polybrene (Invitrogen, Carlsbad, CA, USA). Transfection efficacy was examined at 24 h by quantitative real-time polymerase chain re- action (qRT-PCR). Search results for lipofectamine at Sigma-Aldrich. Compare Products: Select up to 4 products. *Please select more than one item to compare I would like to transfect 293T cells with Lipofectamine 3000. This is the first time I will have used Lipofectamine 3000, previously I only used Lipofectamine 2000, and I am wondering if anyone ... The let-7 mutant is lethal in the nematode (Reinhart et al., 2000), and decreased let-7 expression or genomic deletion has been detected in several human cancer types (Takamizawa et al., 2004; Dahiya et al., 2008; O’Hara et al., 2009). [score:3] Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. Figure 2. The transfection protocol for Lipofectamine 3000 was developed to be easy to use while still ensuring optimum performance and reliability in a wide panel of cell lines. When the cells reached 50% confluence, 100 nM of ATM-siRNA or Chk2-siRNA was transfected with 5 μL of Lipofectamine 2000 plus 1.5 mL of serum-free RPMI-1640 medium without antibiotics following the instructions described by the manufacturer. International, LLC. Lipofectamine 2000 plusTM (Invitrogen) was used as a transfection reagent in serum-free DMEM as described previously (9). Forty-eight hours after the transfec-tion, cells were used for further experiments. ROS Production—O 2. production by homogenates of cul-tured cells was measured using lucigenin (5 M)-enhanced 生物在线 > 产品库 >试剂库 > "Cytotoxicity Det.Kit PLUS ... Lipofectamine™ 2000 转染试剂（lip 2000） ...
Use 0.15 μl/well lipofectamine 2000 diluted in 10 μl/well Opti‐MEM. Mix by pipetting and incubate for 5 to 10 min at room temperature. 4. Mix siRNA and Lipofectamine 2000 dilutions (15 + 10 = 25 μl/well). 5. Incubate at room temperature for 20 min. 6. Add 25 μl/well siRNA and Lipofectamine 2000 transfection mix to cell plates. 7. (Origene) using the Lipofectamine 2000 Plus reagent (Invitrogen), and transfected cells were then selected in the presence of 400 mg/mL G418 to generate the control and GFP-TLE1 pools as described above. In transient transfec-tion assays, the total amount of plasmid used per transfec-tion was normalized with the corresponding empty vector constructs.
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Enzyme transduction was performed 6 h after plating using Lipofectamine LTX (Invitrogen), Lipofectamine 3000 (Invitrogen) and Proteofectene (Biontex). Proteins were pre-incubated for 20 minutes at 20 °C in 100 μl Optimem (Gibco) with or without 2.5 μl of the transfection reagents, with 10 μl (for 96-well) or 30 μl (for 48-well) being added ... Mix Lipofectamine 2000 gently before use, then dilute 1 μl in 50 μl Opti- MEM I Reduced Serum Medium. Mix gently and incubate for 5 minutes at room temperature. Note: Proceed to Step c within 25 minutes. c. After the 5-minute incubation, combine the diluted oligomer with the diluted Lipofectamine 2000. Mix gently and incubate for 20 minutes ...货号： 13778075 规格 Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells Sample Type (Specific): Synthetic siRNA Transfection Technique: Lipid-Based Transfection Ser Each condition (well) contained 4 μg of pCMV-CΔ553 or empty vector pCMV-Tag4A per 7.5 μl of Lipofectamine 2000. RT/real-time QPCR results showing the amount of messages from various conditions normalized to the amount of messages in the standard (minus CΔ553 plus inducer) which is arbitrarily set to one in all cases (first left column in ... 2 ug GFP-dynamitin plasmid using Lipofectamine 2000 Plus (Invitrogen) according to manufacturers instructions. Briefly, 2 ug of plasmid was diluted in 250 ul of MEM mixed with 250 ul of MEM containing 10 ul of Lipofectamine and incubated for 30 min at RT. The DNA-liposome complex was then added to preformed monolayer of BHK-21 cells with 70% ... Cells were transfected using Lipofectamine 2000 (KLC-mCit, all FLAG-JIP3 and GFP-JIP3 constructs) or Lipofectamine 3000 (FLAG-KHC), both from Invitrogen, according to the manufacturer's instructions. .. Twenty-four hours after transfection, COS7 cells were lysed in ice-cold buffer (500 m m PIPES, 1 m m EGTA, 1 m m MgSO4 , 0.1% Triton X-100 ...