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When cells reached 80% confluent, they were transfected with SOCS-3 expression plasmids using Lipofectamine 2000 plus reagent according to the manufacturer’s instructions. PRK5 were used as a control. Successful transfection of SOCS-3 genes into trophoblasts was confirmed by positive green fluorescence reviewed under fluorescent microscope. Lipofectamine® 2000 Reagent Protocol 2013-2-Lipofectamine® 2000 DNA Transfection Reagent Protocol Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations. 上海代轩生物科技有限公司发布在丁香通的Lipofectamine® 3000 Transfection Reagent报价、型号、品牌等供应信息介绍,丁香通致力为您提供最优质的Lipofectamine® 3000 Transfection Reagent厂商信息。